What units are typically used to measure microorganisms?
Know the general difference in the types of light microscopy (compound light microscopy, darkfield microscopy, phase-contrast microscopy, differential interference contrast (DIC) microscopy, fluorescence microscopy, and confocal microscopy).
How do you determine the total magnification when using a compound light microscope?
Define resolution
Define refractive index
Understand immunofluorescence or the fluorescent-antibody technique.
Differentiate transmission electron microscopy (TEM) from scanning electron microscopy (SEM).
What are the basic steps when preparing a sample for light microscopy observation?
What is the difference between a basic and an acidic dye and when would each be used?
What is simple staining?
Define mordant
What is differential staining? Be familiar with the basic steps for the gram stain and the acid-fast stain.
Be familiar with the special stains presented – negative staining for capsules, endospore staining and flagella staining.
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